In this category, the Back in Time glitch, and all its derivatives, are banned. All other glitches are allowed. This also means the file setup doesn't matter; all you need is a blank Hero Mode file in one of the three slots.
Your blank Hero Mode file needs at least one Ancient Flower (to repair Scrapper). Upgrading to the Quick Beetle is slower, but if you want to do the upgrade anyway, you'll need as well:
You must choose to perform either Rope Floria or Fence Hop later in the run. This choice changes the route slightly.
Rope Floria Route (faster):
Fence Hop Route (slower):
Routes merge here
Ancient Cistern has two routes depending on the version you're running. Both possible routes follow.