It is possible (and necessary in the current any% route) to skip killing one of the skulltulas on the first set of vines in the first room. To do it, kill a Deku Baba, and throw the Deku Nut at the lower skulltula on the right (GCN)/ left (Wii). It is possible to skip both, but it is incredibly difficult and requires a huge amount of luck manipulation.
The fastest way to kill the giant skulltulas varies depending on whether you have the Master Sword or not. With it, a single spin attack should suffice. Without it, simply add in a jumpslash before the spin attack. Note that if the spin attack does not hit twice, the skulltula will survive.
Roll to the side of the skulltula. You can normally grab the ledge and pull yourself up, but you can add a jumpslash at the end to save time.
Go to the area that would be your path back from Ook. Get on the edge of near the first rotating platform and cue Midna (and exit out of her text box) to switch the wind and have the bridge turn. As it is turning towards you, walk forward to clip into the bridge's railing. Cue Midna to turn the bridge again, jump forward and use a jumpslash to land on the second bridge. You should have a clear path to the area of land with the fifth monkey.
Note: Although standing at the left, right, or middle of the platform before the first rotating bridge will work for clipping, the timing for standing in the middle is the most forgiving.
For the second pair of rotating bridges, head to the edge of the first bridge, position yourself towards the right corner (GCN, or left Wii) at a 45 degree angle and wait for the wind to turn. Just after the first railing of the bridge passes you, jump to clip into the second bridge. A well timed sidehop will also work for this.
Note for Individual Level Runners: Skipping the first cutscene in this room on the first floor (via the clawshot) will cause malfunctions with the camera in this room, causing it to pan into the ground every time you cue Midna or press L (GCN, or Z on Wii).
To skip killing the first Deku Like, jump off the ledge on the right side angled slightly to the right. If you do not jump from the right place, you will be swallowed by the Deku Like. Note that GCN users will be jump off the left, angled slightly to the left.
The major Deku Baba can be defeated by using jumpslashes when it is rearing up to attack. After it is defeated, jumpslash at one of the bomblings before the cutscene, as fuses do not move during cutscenes. Finally, once the bombling is in the base's mouth, push yourself up against the north side of the Deku Base, on the leaf. Once it dies, you can grab the small key without moving.
Grab the bombling outside the room with the fourth monkey. While holding it, have link grab on to the ledge, wait a bit, then drop down. Because grabbing the ledge makes Link drop the bombling, you will drop down, save from harm, saving a couple seconds.
Climb on to the ledge next to the stairs, and line yourself up with the the fencepole between you and the ledge past the fence. Roll forward, and continue holding forward. Link should roll off the pole and directly on to the ledge.
Jump into the water and grab the platform the deku like is on to pull yourself up. Now when Link pulls himself up onto it, he will experience a few frames after his "climbing onto the platform" animation where he stands up completely before falling back into the water. The first visual frame that you see Link stand up, hold the control stick towards the small key chest and mash A. When done correctly, you will jump to the platform every time.
Note: The front left corner of the deku like platform (the lightest one nearest the chest) will give you the best position relative to the chest and the longest jump. Also, remember not to continue to hold forward on the control stick after you have jumped to avoid rolling past or into the chest after hitting the platform.
After the boulder at the top of the vines is destroyed, bring the bombling over to you. Set it down, the aim at the roof of the area with the bommerand, then lock (with the same throw) just above the area containing the monkey. Release the boomerang, and the bombling should hit the wall and just barely blow up the rock.
Drop down the first set of vines, and draw your sword. Jump towards the first bridge, adding in a jumpslash to make the jump.
Open up the holes to your left and right (on Wii, this room is skipped on GCN), and then line yourself pretty much directly opposite with the door. Aim down the hole, and try to find something you can lock onto (yellow revolving cursor). Lock on, and the boomerang should bring up a bombling. Drop it down the other hole, it will blow up the cage. Please note that you must be very precise to find the bombling.
Open up only the right hole, and align yourself much in the same way. After you lock and throw the boomerang, quickly roll over to the hole where you would normally fall down and stand on the opposite side of it. The boomerang should bring the bombling back and hit the cage on its way through, destroying it. As with Paraxade's method, precision is necessary.
As you enter the room, there is a tile worm just in front of you to your right. Walk onto the right side of it. It will boost you up onto the upper platform. Jump across to the other platform.
Now, to rescue the monkey, roll right next to the hanging deku baba. QUICKLY pull out your boomerang, target both fans, release it, and roll away. Try to jump back to the tile worm area. The monkey will be free, and you are very close to the door.
Using the Gale Boomerang, Long Jump Attack over to the monkey tree. Climb the left side of the tree using jump slashes, and then use another Long Jump Attack to jump to the door.
Rolling before the cutscene where the monkeys form your swing across allows you to get close enough to get across the very first time they come back to your side. If you miss the roll, you must wait for them to do an additional swing.
By grabbing Ook's first bomb and placing it on the ground, then hitting Diababa with it, Ook will bring out a second bomb immediately.